"Do my qPCR calculation" can automatically process qPCR raw data (Cq) to get the data normalization and graphical representation of the different samples in an Excel file (readable via Microsoft Office, LibreOffice...). This tool is also available on Github for installation and local use with or without web interface at

Please cite "Do my qPCR calculation":
Tournayre, J., Reichstadt, M., Parry, L., Fafournoux, P., and Jousse, C. (2019). “Do my qPCR calculation”, a web tool. Bioinformation 15, 369–372. Link to the article
  Example:   Without reference gene:   With multiple reference genes:

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This tool is developed and maintained by Team Proteostasis1 and Team PIGALE2

1Université Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France
2Université Clermont Auvergne, VetAgro Sup, INRA, UMR1213, Unité Mixte de Recherche sur les Herbivores, Clermont-Ferrand, France